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KMID : 0613820120220040456
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Journal of Life Science
2012 Volume.22 No. 4 p.456 ~ p.465
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Establishment of a Stable Cell Line Expressing Human BMP2/7-PTD for Efficient Osteogenic Induction
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Park Seung-Won
Kang Seok-Woo
Goo Tae-Won
Kim Seong-Ryul
Paik Soon-Young
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Abstract
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Heterodimeric recombinant human bone morphogenetic proteins (rhBMPs) are powerful tools for bone tissue engineering. However, BMPs have several important limitations in their application to bone regeneration. BMPs have a short half-life and must be used in high concentrations, which may be cost-inefficient. To overcome these problems, we established a stable cell line that expressed the fusion protein comprised of recombinant human BMP2/7 heterodimer protein and PTD (rhBMP2/7-PTD). This stable cell line enabled high process yields by continuously expressing rhBMP2/7-PTD products at high levels throughout cultivation. This synthesized BMP7 was fused to a BMP2 protein with four glycine residues (to allow free bond rotation of the domains) and PTD. To demonstrate that the rhBMP2/7-PTD protein that was secreted from an rhBMP2/7-PTD-expressing stable cell line exhibited biological activity consistent with its role as an osteogenic differentiation induction growth factor, we evaluated BMP-induced ALP activity. Our results suggest that this cell line may be a powerful and efficient tool for applications such as bone tissue regeneration.
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KEYWORD
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Bone morphogenetic protein, protein transduction domain, stable cells, bone tissue regeneration
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